Jundishapur Journal of Health Sciences

Published by: Kowsar

Fraud Identification of Cow’s Milk in Buffalo’s Milk and It’s Products Using the Polymerase Chain Reaction

Mehdi Zarei 1 , * , Siavash Maktabi 1 and Mojtaba Nasiri 1
Authors Information
1 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Health Sciences: October 2016, 8 (4); e60326
  • Published Online: September 5, 2016
  • Article Type: Research Article
  • Received: January 28, 2016
  • Revised: February 29, 2016
  • Accepted: March 2, 2016
  • DOI: 10.17795/jjhs-36555

To Cite: Zarei M, Maktabi S, Nasiri M. Fraud Identification of Cow’s Milk in Buffalo’s Milk and It’s Products Using the Polymerase Chain Reaction, Jundishapur J Health Sci. 2016 ; 8(4):e60326. doi: 10.17795/jjhs-36555.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Bottero M, Civera T, Nucera D, Rosati S, Sacchi P, Turi R. A multiplex polymerase chain reaction for the identification of cows', goats' and sheep's milk in dairy products. Int Dairy J. 2003; 13(4): 277-82[DOI]
  • 2. Calvo J, Osta R, Zaragoza I, Zaragoza P. Species-specific amplification for detection of bovine, ovine and caprine cheese. Milchwissenschaft. 2002; 57(8): 444-6
  • 3. Di Pinto A, Conversano MC, Forte VT, Novello L, Tantillo G. Detection of cow milk in buffalo "mozzarella" by polymerase chain reaction (PCR) assay. J Food Quality. 2004; 27(6): 428-35[DOI]
  • 4. Lopez-Calleja I, Gonzalez I, Fajardo V, Rodriguez MA, Hernandez PE, Garcia T, et al. Rapid detection of cows' milk in sheeps' and goats' milk by a species-specific polymerase chain reaction technique. J Dairy Sci. 2004; 87(9): 2839-45[DOI][PubMed]
  • 5. Abdel-Rahman S, Ahmed M. Rapid and sensitive identification of buffalo's, cattle's and sheep's milk using species-specific PCR and PCR-RFLP techniques. Food Control. 2007; 18(10): 1246-9[DOI]
  • 6. Branciari R, Nijman IJ, Plas ME, Di Antonio E, Lenstra JA. Species origin of milk in Italian mozzarella and Greek feta cheese. J Food Prot. 2000; 63(3): 408-11[PubMed]
  • 7. Mafra I, Ferreira IM, Faria MA, Oliveira BP. A novel approach to the quantification of bovine milk in ovine cheeses using a duplex polymerase chain reaction method. J Agric Food Chem. 2004; 52(16): 4943-7[DOI][PubMed]
  • 8. Rea S, Chikuni K, Branciari R, Sangamayya RS, Ranucci D, Avellini P. Use of duplex polymerase chain reaction (duplex-PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese. J Dairy Res. 2001; 68(4): 689-98[PubMed]
  • 9. Murphy MA, Shariflou MR, Moran C. High quality genomic DNA extraction from large milk samples. J Dairy Res. 2002; 69(4): 645-9[PubMed]
  • 10. Sambrook J, Russell David W. Molecular cloning: a laboratory manual. 1989; 3
  • 11. Ghovvati S, Nassiri MR, Mirhoseini S, Moussavi AH, Javadmanesh A. Fraud identification in industrial meat products by multiplex PCR assay. Food Control. 2009; 20(8): 696-9[DOI]
  • 12. Haunshi S, Basumatary R, Girish PS, Doley S, Bardoloi RK, Kumar A. Identification of chicken, duck, pigeon and pig meat by species-specific markers of mitochondrial origin. Meat Sci. 2009; 83(3): 454-9[DOI][PubMed]
  • 13. Kesmen Z, Sahin F, Yetim H. PCR assay for the identification of animal species in cooked sausages. Meat Sci. 2007; 77(4): 649-53[DOI][PubMed]
  • 14. Rodriguez MA, Garcia T, Gonzalez I, Asensio L, Hernandez PE, Martin R. PCR identification of beef, sheep, goat, and pork in raw and heat-treated meat mixtures. J Food Prot. 2004; 67(1): 172-7[PubMed]
  • 15. Rodriguez MA, Garcia T, Gonzalez I, Asensio L, Mayoral B, Lopez-Calleja I, et al. Identification of goose, mule duck, chicken, turkey, and swine in foie gras by species-specific polymerase chain reaction. J Agric Food Chem. 2003; 51(6): 1524-9[DOI][PubMed]
  • 16. Khanzadi S, Jamshidi A, Razmyar J, Mohsenzadeh M. PCR-based detection of cow and goat milk in sheep milk and dairy products marketed in Mashhad city of Iran. Iranian J Veter Med. 2013; 7(4): 257-62
  • 17. Maudet C, Taberlet P. Detection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism. J Dairy Res. 2001; 68(2): 229-35[PubMed]
  • 18. Stanciuc N, Rapeanu G. Identification of adulterated sheep and goat cheeses marketed in Romania by immunocromatographic assay. Food Agriculture Immunol. 2010; 21(2): 157-64[DOI]
  • 19. Maskova E, Paulickova I. PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic. Czech Journal of Food Science Vol. 2006; 24(3): 127-32
  • 20. Santos J, Fernandes P, Bardsley R. Portuguese "PDO "cheese and species origin of milk. Electro J Environmet. 2003; 2: 476-9
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments