Jundishapur Journal of Health Sciences

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Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef

Siavash Maktabi 1 , * , Mehdi Zarei 1 and Hooriyeh Mohammadpour 2
Authors Information
1 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
2 Graduated from Department of Food Hygiene, faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Health Sciences: January 01, 2016, 8 (1); e31195
  • Published Online: January 9, 2016
  • Article Type: Research Article
  • Received: August 23, 2015
  • Revised: November 1, 2015
  • Accepted: November 2, 2015
  • DOI: 10.17795/jjhs-31195

To Cite: Maktabi S, Zarei M, Mohammadpour H. Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef, Jundishapur J Health Sci. 2016 ; 8(1):e31195. doi: 10.17795/jjhs-31195.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Williams RC, Isaacs S, Decou ML, Richardson EA, Buffett MC, Slinger RW, et al. Illness outbreak associated with Escherichia coli O157:H7 in Genoa salami. E. coli O157:H7 Working Group. CMAJ. 2000; 162(10): 1409-13[PubMed]
  • 2. Jay JM. Food Microbiology. 1992; [DOI]
  • 3. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, et al. Food-related illness and death in the United States. Emerg Infect Dis. 1999; 5(5): 607-25[DOI][PubMed]
  • 4. Cagney C, Crowley H, Duffy G, Sheridan JJ, O’Brien S, Carney E, et al. Prevalence and numbers of Escherichia coli O157: H7 in minced beef and beef burgers from butcher shops and supermarkets in the Republic of Ireland. Food Microbiol. 2004; 21(2): 203-12
  • 5. Ahmed AM, Shimamoto T. Isolation and molecular characterization of Salmonella enterica, Escherichia coli O157:H7 and Shigella spp. from meat and dairy products in Egypt. Int J Food Microbiol. 2014; 168-169: 57-62[DOI][PubMed]
  • 6. Fantelli K, Stephan R. Prevalence and characteristics of shigatoxin-producing Escherichia coli and Listeria monocytogenes strains isolated from minced meat in Switzerland. Int J Food Microbiol. 2001; 70(1-2): 63-9[PubMed]
  • 7. Sarimehmetoglu B, Aksoy MH, Ayaz ND, Ayaz Y, Kuplulu O, Kaplan YZ. Detection of Escherichia coli O157: H7 in ground beef using immunomagnetic separation and multiplex PCR. Food Control. 2009; 20(4): 357-61
  • 8. Varela-Hernandez JJ, Cabrera-Diaz E, Cardona-Lopez MA, Ibarra-Velazquez LM, Rangel-Villalobos H, Castillo A, et al. Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 from beef carcasses at a slaughter plant in Mexico. Int J Food Microbiol. 2007; 113(2): 237-41[DOI][PubMed]
  • 9. Ekiri AB, Landblom D, Doetkott D, Olet S, Shelver WL, Khaitsa ML. Isolation and characterization of shiga toxin-producing escherichia coli serogroups O26, O45, O103, O111, O113, O121, O145, and O157 shed from range and feedlot cattle from postweaning to slaughter. J Food Prot. 2014; 77(7): 1052-61[DOI][PubMed]
  • 10. First Isolation of Shiga Toxin-producing Escherichia coli O157: H7 in Ground Beef at Retail Market in Sao Paulo City, Brazil. Annual Meeting.
  • 11. Xu JG, Quan TS, Xiao DL, Fan TR, Li LM, Wang CA. Isolation and characterization ofEscherichia coli O157:H7 strains in China. Current Microbio. 1990; 20(5): 299-303
  • 12. Jafareyan-Sedigh M, Rahimi E, Doosti A. Isolation of Escherichia coli O157: H7 in sheep meats using cultural and PCR method. J Shahrekord U Med Sci. 2011; 13(2): 61-8
  • 13. Shekarforoush S, Tahamtan Y, Pourbakhsh A. Detection and frequency of Stx2 gene in Escherichia coli O157 and O157:H7 strains isolated from sheep carcasses in Shiraz-Iran. Pak J Biol Sci. 2008; 11(8): 1085-92[PubMed]
  • 14. Shahrokhabadi R, Rahimi E, Momtaz H, Poursahebi R. Identification of Escherichia coli O157: H7 on Bovine Carcasses in Rafsanjan by Using Culture and PCR Method . 2012;1(8):49-53. J Veterinary Microbiol. 2012;
  • 15. Gyles CL. Shiga toxin-producing Escherichia coli: an overview. J Anim Sci. 2007; 85(13 Suppl)-62[DOI][PubMed]
  • 16. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998; 11(1): 142-201[PubMed]
  • 17. Karmali MA. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 1989; 2(1): 15-38[PubMed]
  • 18. March SB, Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol. 1986; 23(5): 869-72[PubMed]
  • 19. Carney E, O'Brien SB, Sheridan JJ, McDowell DA, Blair IS, Duffy G. Prevalence and level of Escherichia coli O157 on beef trimmings, carcasses and boned head meat at a beef slaughter plant. Food Microbiol. 2006; 23(1): 52-9[DOI][PubMed]
  • 20. Lopez-Saucedo C, Cerna JF, Villegas-Sepulveda N, Thompson R, Velazquez FR, Torres J, et al. Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli. Emerg Infect Dis. 2003; 9(1): 127-31[DOI][PubMed]
  • 21. Paton AW, Paton JC. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J Clin Microbiol. 1998; 36(2): 598-602[PubMed]
  • 22. Gannon VP, D'Souza S, Graham T, King RK, Rahn K, Read S. Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J Clin Microbiol. 1997; 35(3): 656-62[PubMed]
  • 23. Gannon VP, King RK, Kim JY, Thomas EJ. Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction. Appl Environ Microbiol. 1992; 58(12): 3809-15[PubMed]
  • 24. Lee GY, Jang HI, Hwang IG, Rhee MS. Prevalence and classification of pathogenic Escherichia coli isolated from fresh beef, poultry, and pork in Korea. Int J Food Microbiol. 2009; 134(3): 196-200[DOI][PubMed]
  • 25. Banatvala N, Griffin PM, Greene KD, Barrett TJ, Bibb WF, Green JH, et al. The United States National Prospective Hemolytic Uremic Syndrome Study: microbiologic, serologic, clinical, and epidemiologic findings. J Infect Dis. 2001; 183(7): 1063-70[DOI][PubMed]
  • 26. Vernozy-Rozand C, Ray-Gueniot S, Ragot C, Bavai C, Mazuy C, Montet MP, et al. Prevalence of Escherichia coli O157:H7 in industrial minced beef. Lett Appl Microbiol. 2002; 35(1): 7-11[PubMed]
  • 27. Chinen I, Tanaro JD, Miliwebsky E, Lound LH, Chillemi G, Ledri S, et al. Isolation and characterization of Escherichia coli O157:H7 from retail meats in Argentina. J Food Prot. 2001; 64(9): 1346-51[PubMed]
  • 28. Samadpour M, Kubler M, Buck FC, Depavia GA, Mazengia E, Stewart J, et al. Prevalence of Shiga toxin-producing Escherichia coli in ground beef and cattle feces from King County, Washington. J Food Prot. 2002; 65(8): 1322-5[PubMed]
  • 29. Tarr PI, Tran NT, Wilson RA. Escherichia coli O157:H7 in retail ground beef in Seattle: results of a one-year prospective study. J Food Prot. 1999; 62(2): 133-9[PubMed]
  • 30. Rahimi E, Kazemeini HR, Salajegheh M. Escherichia coli O157:H7/NM prevalence in raw beef, camel, sheep, goat, and water buffalo meat in Fars and Khuzestan provinces, Iran. Vet Res Forum. 2012; 3(1): 15-7[PubMed]
  • 31. Momtaz H, Safarpoor Dehkordi F, Rahimi E, Ezadi H, Arab R. Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant's meat. Meat Sci. 2013; 95(2): 381-8[DOI][PubMed]
  • 32. Wang L, Rothemund D, Curd H, Reeves PR. Sequence diversity of the Escherichia coli H7 fliC genes: implication for a DNA-based typing scheme for E. coli O157:H7. J Clin Microbiol. 2000; 38(5): 1786-90[PubMed]
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